Anti-CREB5 Rabbit Polyclonal Antibody for WB and ELISA - Q02930

Core Product Specifications and Parameters

Biological Background: CREB5 Function and Localization

• CREB5 (Cyclic AMP-responsive element-binding protein 5), also known as CRE-BPa, is a transcription factor that recognizes the cAMP response element (CRE).
• It functions as a transcriptional activator by binding specifically to CRE sequences in the promoter regions of target genes.
• The protein localizes predominantly to the nucleus, consistent with its role in gene regulation.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation), which may modulate its activity and stability.
• CREB5 contains zinc-finger motifs and participates in metal-ion binding, likely aiding DNA interaction.
• It has been identified in proteomics studies and is part of the human reference proteome.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 57 kDa in human samples; minor bands from alternative splice variants may appear.
• Use nuclear-enriched tissue or cell lysates to improve detection of this transcription factor.
• In ELISA, optimize coating using recombinant CREB5 protein or a peptide spanning amino acids 210–380.
• Validate cross-reactivity with rat samples using tissues like brain or testis, where CREB5 is conserved.
• As a polyclonal antibody, verify lot-to-lot consistency for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with premium IVD raw materials like this CREB5 antibody, supporting assay development in transcription factor research and signal transduction studies. Beyond single antibodies, we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents for your entire workflow. Our supply chain expertise ensures reliable access to rare targets and custom sourcing, streamlining your path from concept to clinic.