Anti-CREB3L2 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q70SY1

Core Product Specifications and Parameters

Biological Background: CREB3L2 Function and Localization

• CREB3L2, also known as BBF2H7, is a transcription factor belonging to the CREB/ATF family that mediates the unfolded protein response (UPR).
• Under basal conditions, CREB3L2 is anchored in the endoplasmic reticulum (ER) membrane, with its N‑terminal transcription activation and DNA‑binding domains facing the cytosol.
• Endoplasmic reticulum stress triggers transport to the Golgi, where regulated intramembrane proteolysis by S1P/MBTPS1 and S2P/MBTPS2 releases the N‑terminal fragment for nuclear import and gene activation.
• In vitro, the released N‑terminal fragment binds directly to CRE sites and activates transcription of target genes.
• Related references: PMID:17178827
• In a neuroblastoma cell model, CREB3L2 protects cells from ER stress‑induced apoptosis.
• Related references: PMID:17178827
• CREB3L2 plays a critical role in chondrogenesis by activating SEC23A transcription, which promotes the secretion of cartilage matrix proteins (By similarity).
• Subcellularly, the full‑length protein resides in the ER membrane, while the cleaved active form translocates to the nucleus to regulate gene expression.
• CREB3L2 is widely expressed, with highest levels in placenta, lung, spleen, and intestine, and lowest in heart, brain, and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (aa 1‑247) of human CREB3L2, encompassing the transcription activation and DNA‑binding domains; this region is retained in the cleaved active form, so the antibody may detect both full‑length (57 kDa) and cleaved N‑terminal fragments in WB under reducing conditions.
• For WB, consider using cultured cell lines exposed to ER stress inducers (e.g., tunicamycin) to monitor CREB3L2 cleavage and activation.
• In IHC‑P, expect nuclear staining after ER stress activation; validate in tissues with known high expression such as placenta or lung.
• For IF/ICC, permeabilization is required to access the cytosolic/nuclear epitope; optimize fixation and permeabilization conditions.
• As a rabbit monoclonal antibody, it offers high specificity and batch‑to‑batch consistency; however, we recommend titrating the antibody for each assay to determine the optimal working dilution.

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