Anti-CREB1 Rabbit Polyclonal Antibody for WB, IP, ELISA - P16220

Core Product Specifications and Parameters

Biological Background: CREB1 Function and Localization

• CREB1 (Cyclic AMP-responsive element-binding protein 1) is a phosphorylation-dependent transcription factor that binds to cAMP response elements (CRE) in DNA, activating transcription. TORC coactivators can enhance this activation independently of Ser-119 phosphorylation. Related references: PMID:14536081
• It plays a critical role in synchronizing circadian rhythmicity and regulating the differentiation of adipose cells.
• In cardiomyocytes, CREB1 regulates the expression of apoptotic and inflammatory response factors in response to ERFE-mediated activation of AKT signaling.
• The protein predominantly localizes to the nucleus to exert its transcriptional regulatory functions.
• CREB1 undergoes various post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which modulate its activity.
• Alternative splicing generates isoforms, and CREB1 is also involved in host-virus interactions and chromosomal rearrangements.
• Commonly referred to as CREB or CREB-1, this protein is a key node in cellular signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 1-120 of human CREB1; this N-terminal region may contribute to the antibody's specificity. Ensure that the immunogen sequence matches your target region of interest.
• For Western blot, the expected molecular weight is approximately 35 kDa; however, post-translational modifications may cause slight variations in migration patterns. Validate the observed band size in your sample system.
• This antibody has been used in immunoprecipitation (IP) assays; consider optimizing antibody concentration and incubation conditions for best results.
• Cross-reactivity is reported for human, mouse, and rat, but it is advisable to confirm reactivity in your particular experimental models and applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this CREB1 polyclonal antibody as part of a broad portfolio for transcription and cell signaling research. Our platform facilitates efficient procurement of validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing, supporting diagnostic manufacturers and research labs from initial concept through clinical application.