Anti-CREB1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P16220

Core Product Specifications and Parameters

Biological Background: Cyclic AMP-responsive element-binding protein 1 Function and Localization

• Cyclic AMP-responsive element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor that binds to cAMP response elements (CRE) in promoters to stimulate transcription of target genes.
• CREB1 is involved in diverse cellular processes such as synchronization of circadian rhythmicity, differentiation of adipose cells, and regulation of apoptotic and inflammatory responses in cardiomyocytes.
• Transcription activation by CREB1 is enhanced by TORC coactivators, which act independently of Ser-119 phosphorylation. Related references: PMID:14536081
• CREB1 belongs to the CREB/ATF family of bZIP transcription factors and is subject to alternative splicing, generating multiple isoforms.
• The protein localizes predominantly to the nucleus.
• CREB1 undergoes several post‑translational modifications including phosphorylation, ubiquitination, and isopeptide bond formation (SUMOylation), as indicated by UniProt keywords.
• Additional keywords highlight its roles in biological rhythms, chromosomal rearrangements, host‑virus interactions, and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant protein spanning amino acids 1–341 of human CREB1, covering the full‑length sequence, which may facilitate recognition of multiple splice variants and phosphorylated forms.
• For Western blot, consider enriching nuclear fractions to improve detection, given the nuclear localization of CREB1.
• All listed applications (WB, IHC-P, IF/ICC, IP, ELISA) should be individually validated in the relevant sample system, particularly paying attention to potential cross‑reactivity with other CREB/ATF family members.
• IHC-P and IF/ICC experiments may benefit from optimized antigen retrieval and careful fixation methods to preserve epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw materials, providing validated polyclonal antibodies like this anti‑CREB1 antibody alongside matched antibody pairs, bulk ancillary reagents, and custom sourcing for hard‑to‑find targets. With a focus on transcription and cell signaling research, CamelBio supports diagnostic manufacturers and laboratories in accelerating assay development from concept to clinic.