Anti-Creatine Kinase M-type (CKM) Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P06732

Core Product Specifications and Parameters

Biological Background: CKM (Creatine Kinase M-type) Function and Localization

• Creatine kinase M-type (CKM), also referred to as CKMM, M-CK, or CPK-M, is a key enzyme in cellular energy homeostasis.
• It reversibly catalyzes the transfer of phosphate between ATP and creatine phosphate, providing a critical energy buffer in tissues with high and fluctuating energy demands.
• CKM is highly expressed in skeletal muscle, heart, and to a lesser extent in brain and spermatozoa, where it maintains local ATP levels during contraction and other energy-intensive processes.
• The enzyme localizes predominantly to the cytoplasm, where it couples ATP production with energy utilization.
• As a member of the creatine kinase family, CKM is classified as a transferase and kinase, featuring ATP- and nucleotide-binding domains.
• Post-translational modifications including phosphorylation have been documented, potentially regulating its activity or localization.

Experimental Guidance and Technical Tips

• The immunogen spans nearly full-length human CKM (aa 3–381), ensuring recognition of multiple epitopes in the native protein. Consider using positive controls such as human skeletal muscle or heart lysates for WB.
• For western blot (WB), a band at approximately 43 kDa is expected. Optimize antibody dilution and exposure times based on sample type and expression levels.
• In immunofluorescence on paraffin-embedded (IF-P) sections, pre-validate the antibody in a tissue known to express CKM (e.g., skeletal muscle) and include appropriate fixation and antigen retrieval steps.
• For ELISA applications, pair this polyclonal antibody with a suitable monoclonal capture antibody if designing a sandwich assay, and validate sensitivity with recombinant CKM protein standards.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material provider, CamelBio delivers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research labs worldwide. With deep expertise in energy metabolism and muscle disease-related targets like CKM, we help you accelerate assay development from concept to clinic. Explore our catalog for rare-target sourcing and flexible supply solutions tailored to your project needs.