Anti-Creatine kinase B type (CKB) Monoclonal Antibody for WB, IHC-P, ELISA - P12277

Core Product Specifications and Parameters

Biological Background: Creatine kinase B-type Function and Localization

• Creatine kinase B-type (CKB) is a member of the creatine kinase family, encoding the brain/brain-type isoform (B-CK), which forms homodimers (CKBB) or heterodimers with muscle-type CKM (CKMB).
• Reversibly catalyzes transfer of phosphate between ATP and creatine, producing phosphocreatine and ADP, crucial for energy homeostasis in high-demand tissues (PubMed:8186255). Related references: PMID:8186255
• Functions in the futile creatine cycle in thermogenic adipocytes, dissipating energy as heat by cycling creatine and phosphocreatine (By similarity).
• Localizes to cytosol, but also associates with mitochondria and cell membrane, supporting subcellular energy channeling.
• Post-translational modifications include phosphorylation, nitration, isopeptide bond formation, and ubiquitination, potentially regulating activity and stability.
• Keywords: kinase, transferase, ATP-binding, nucleotide-binding, membrane, mitochondrion, phosphoprotein, Ubl conjugation.
• May also play a role in brain development and sperm motility, consistent with expression in brain and testis.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~43 kDa; use reducing SDS-PAGE and validate with positive control lysates from brain, heart, or skeletal muscle.
• For IHC-P, optimize antigen retrieval and use sections from human brain or muscle as positive controls; cross-reactivity with mouse and rat enables validation in animal models.
• ELISA: synthetic peptide immunogen suggests direct binding capability; consider pairing with another anti-CKB antibody targeting a distinct epitope for sandwich ELISA development.
• Because CKB localizes to both cytoplasm and mitochondria, subcellular fractionation or mitochondrial isolation may be useful to confirm localization-specific signals.
• Species cross-reactivity (human, mouse, rat) is indicated; verify reactivity in your specific sample type and species with appropriate secondary antibodies and controls.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio provides global diagnostic manufacturers and research teams with reliable, scalable access to high-quality antibodies like this anti-CKB monoclonal antibody, supporting energy metabolism, thermogenesis, and neuromuscular disease research. Beyond individual reagents, CamelBio streamlines sourcing for validated antibody pairs, bulk ancillary reagents, and rare target raw materials—all designed to accelerate your assay development from concept to clinic. Contact us to explore customized procurement solutions that meet your project’s specific needs.