Anti-CRCP Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75575

Core Product Specifications and Parameters

Biological Background: CRCP Function and Localization

• Core subunit of DNA-directed RNA polymerase III (Pol III), catalyzing the transcription of DNA into small non-coding RNAs such as 5S rRNA, tRNAs, and miRNAs. Related references: PMID:20413673 PMID:33558764 PMID:34675218
• Together with subunit POLR3H/RPC8, forms a mobile stalk that protrudes from the Pol III core and is primarily involved in transcription initiation. Related references: PMID:20413673 PMID:33558764 PMID:33558766
• Functions as a nuclear and cytosolic DNA sensor, recognizing non-self dsDNA and playing an important role in innate antiviral immune responses through the RIG-I pathway. Related references: PMID:19609254 PMID:19631370
• Serves as an accessory protein for the calcitonin gene-related peptide (CGRP) receptor, modulating CGRP responsiveness in various tissues.
• Alternative splicing generates multiple isoforms, with tissue-specific expression patterns.
• Localizes to both the nucleus and the cell membrane.
• Ubiquitously expressed, with highest levels observed in testis.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1–115, a region that shares high sequence identity across human, mouse, and rat; consider this when planning multispecies studies.
• For Western blot, the expected molecular weight is approximately 17 kDa under standard reducing conditions; titrate the antibody to minimize background.
• In IHC-P, antigen retrieval may improve detection, especially given the dual nuclear and membrane localization of CRCP.
• ELISA performance can be optimized by using recombinant CRCP or cell lysates; include appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. The CRCP antibody supports research in RNA polymerase III biology, CGRP receptor signaling, and innate immunity. Let our team assist you in sourcing rare targets and scaling your assay development from concept to clinic.