Anti-CRACR2A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BSW2

Core Product Specifications and Parameters

Biological Background: CRACR2A Function and Localization

• CRACR2A, also known as EF-hand calcium-binding domain-containing protein 4B (EFCAB4B) or Ras-related protein Rab-46 (RAB46), is a calcium-binding protein with GTPase activity that plays dual roles in store-operated calcium entry (SOCE) and intracellular trafficking. Related references: PMID:27016526
• In T-cells, CRACR2A functions as a cytoplasmic calcium sensor that facilitates clustering of ORAI1 and STIM1 at ER-plasma membrane junctions, promoting CRAC channel activation. Upon calcium influx, it dissociates from the complex to regulate channel stability. Related references: PMID:27016526
• As a Rab GTPase, CRACR2A mediates retrograde transport of Weibel-Palade bodies (WPBs) to the microtubule organizing center (MTOC) in endothelial cells in response to histamine stimulation, acting in a calcium-independent manner. Related references: PMID:31092558
• CRACR2A also functions as a calcium-regulated dynein adapter protein, activating dynein-mediated transport and regulating endosomal trafficking of CD47. Related references: PMID:30814157
• It bridges TCR signaling pathways by localizing to the immunological synapse upon T-cell activation, where it interacts with VAV1 to activate downstream Ca2+-NFAT and JNK signaling cascades. Related references: PMID:27016526
• Subcellularly, CRACR2A is found in the cytoplasm, at the plasma membrane, Golgi apparatus, trans-Golgi network, and associated with vesicles and the microtubule organizing center. Its localization changes depending on activation state and cell type.
• Tissue expression includes T-cell lines such as Jurkat and endothelial cells, with specific localization to Weibel-Palade bodies in endothelial cells. Related references: PMID:25475730, PMID:31092558, PMID:27016526, PMID:30814157
• The protein contains EF-hand domains for calcium binding, a GTPase domain, and undergoes prenylation (as indicated by keywords), suggesting membrane association. Multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-200) of human CRACR2A, which may target conserved epitopes across isoforms. Consider verifying isoform-specific detection in your experimental system.
• For Western blotting, the predicted molecular weight is approximately 83 kDa. When analyzing endogenous CRACR2A, pay attention to possible post-translational modifications or splice variants that may alter migration.
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized for the specific tissue and antibody binding. Validate staining patterns using appropriate positive (e.g., T-cell-rich tissues) and negative controls.
• The antibody cross-reacts with mouse samples according to supplier specifications, but we recommend validating specificity in mouse tissues or cell lines before use.
• For ELISA applications, consider using a secondary detection system compatible with rabbit polyclonal antibodies; titrate the antibody to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop provider of IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutions from concept to clinic. This anti-CRACR2A polyclonal antibody is part of our extensive portfolio for calcium signaling and T-cell research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, ensuring reliable supply for your assay development needs.