Anti-CRACR2A Rabbit Polyclonal Antibody for WB, ELISA - Q9BSW2

Core Product Specifications and Parameters

Biological Background: CRACR2A Function and Localization

• CRACR2A (EF-hand calcium-binding domain-containing protein 4B), also known as EFCAB4B and RAB46, is a calcium-binding protein that acts as a cytoplasmic calcium-sensor and Rab GTPase.
• Plays a key role in store-operated Ca2+ entry (SOCE) in T-cells by regulating CRAC channel activation, facilitating clustering of ORAI1 and STIM1 at ER-PM junctions. Related references: PMID:27016526
• Functions as a Rab GTPase mediating trafficking of Weibel-Palade bodies to microtubule organizing center in endothelial cells in response to histamine stimulation. Related references: PMID:31092558
• Ca2+-regulated dynein adapter that activates dynein-mediated transport, regulating endosomal trafficking of CD47. Related references: PMID:30814157
• Bridges TCR signaling pathways, linking Ca2+-NFAT and JNK pathways via interaction with VAV1 at the immunological synapse. Related references: PMID:27016526
• Involved in T-helper 1 (Th1) cell differentiation and Th17 effector function. Related references: PMID:29987160
• Localizes to cytoplasm, Golgi apparatus, microtubule organizing center, cell membrane, and vesicles; undergoes alternative splicing and prenylation.
• Expressed in Jurkat T-cell line and endothelial cells, including Weibel-Palade bodies. Related references: PMID:25475730, PMID:27016526, PMID:30814157, PMID:31092558

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-200), which is expected to recognize multiple isoforms; validate in your target sample system.
• For WB, the predicted molecular weight is 83 kDa; consider using fresh lysates from Jurkat cells or endothelial cells.
• Cross-reactivity with human, mouse, and rat has been reported; verify in your experimental models.
• ELISA may be suitable for antibody titer testing or antigen detection; optimize coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including this anti-CRACR2A polyclonal antibody for calcium signaling and T-cell research. Our extensive catalog also offers validated antibody pairs, recombinant proteins, and ancillary reagents for CRAC channel studies. Contact us for bulk orders or customized sourcing of rare-target raw materials.