Anti-CRABP2 Polyclonal Antibody for WB, IF/ICC, ELISA - P29373

Core Product Specifications and Parameters

Biological Background: CRABP2 Function and Localization

• CRABP2 (Cellular retinoic acid-binding protein 2), also known as CRABP-II, is a member of the intracellular lipid-binding protein family.
• It transports retinoic acid from the cytoplasm to the nucleus, thereby regulating the availability of retinoic acid for nuclear retinoic acid receptors (RARs) and modulating retinoic acid signaling pathways.
• The protein is predominantly localized in the cytoplasm but can also be found in the endoplasmic reticulum and nucleus, reflecting its dynamic shuttling role.
• Post-translational modifications include ubiquitination (Ubl conjugation) and isopeptide bond formation, which may influence protein stability or function.
• CRABP2 is involved in vitamin A metabolism and transport, binding all-trans retinoic acid with high affinity.
• It has been identified as a component in various proteomic studies, underscoring its broad cellular relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22–94 of human CRABP2, a region conserved in mouse and rat. For Western blot, expect a band near 16 kDa under reducing conditions; optimize sample preparation accordingly.
• For immunofluorescence/immunocytochemistry, the antibody may detect both cytoplasmic and nuclear pools; ensure fixation and permeabilization protocols preserve subcellular structures.
• In ELISA applications, consider using recombinant CRABP2 protein as a reference standard to assess assay performance and linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined one-stop procurement solution for IVD raw materials, including validated polyclonal antibodies like this anti-CRABP2 antibody, supporting research in retinoic acid signaling, vitamin A metabolism, and cellular transport. Whether you need optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio bridges the gap from concept to clinic for diagnostic manufacturers and laboratories worldwide.