Anti-CRABP1 Monoclonal Antibody for WB, IF-P, ELISA - P29762

Core Product Specifications and Parameters

Biological Background: CRABP1 Function and Localization

• CRABP1, also known as Cellular retinoic acid-binding protein 1 (CRABP-I), is a highly conserved cytoplasmic protein encoded by the CRABP1 gene (Gene ID: 1381).
• It belongs to the intracellular lipid-binding protein family and specifically binds all-trans-retinoic acid, a vitamin A derivative.
• The protein is thought to regulate the availability of retinoic acid to nuclear retinoic acid receptors (RARs and RXRs), thereby influencing retinoid-dependent gene expression.
• CRABP1 is predominantly localized in the cytoplasm, where it may sequester retinoic acid and modulate its concentration.
• It exhibits a molecular weight of approximately 15.6 kDa and adopts a beta-barrel conformation that forms a hydrophobic binding pocket for retinoic acid.
• The protein has been identified through proteomics and structural studies, and it is classified under the retinol-binding protein family.
• CRABP1 plays a role in retinoid metabolism, potentially impacting processes such as cell differentiation and proliferation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 58–137; for western blot, consider testing both reducing and non-reducing conditions to evaluate conformational sensitivity.
• For immunofluorescence (IF-P), validate the antibody in your specific sample system, as CRABP1 is a cytoplasmic target requiring permeabilization.
• For ELISA, optimal coating antigen and antibody dilution should be determined empirically; the antibody may be used in sandwich or competitive formats.
• The antibody shows cross-reactivity with mouse and rat CRABP1; confirm performance in your assay when using rodent samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CRABP1 monoclonal antibody is part of our extensive portfolio of high-quality antibodies for retinoid signaling research. Whether you need validated antibody pairs, bulk ancillary reagents, or rare target raw materials, CamelBio streamlines your sourcing process.