Anti-CR2/CD21 Rabbit Polyclonal Antibody for WB, IHC, IF, ELISA - P20023

Core Product Specifications and Parameters

Biological Background: CR2/CD21 Function and Localization

• Complement receptor type 2 (CR2/CD21) serves as a receptor for complement component C3d, facilitating the recognition and uptake of C3d-opsonized antigens by B-cells, thereby enhancing B-cell activation and immune responses. Related references: PMID:1849076, PMID:21527715, PMID:7753047
• CR2/CD21 forms the B-cell coreceptor complex with CD19, FCRL5, and CD81, which is essential for B-cell signaling and activation. Related references: PMID:1383329, PMID:30107486
• Independently of the B-cell receptor and CD19, CR2/CD21 can activate SRC kinase, leading to phosphorylation of nucleolin and triggering AKT and GSK3 kinase activities. Related references: PMID:12938232
• CR2/CD21 acts as a ligand for CD23 (FcεRII), participating in the regulation of IgE production. Related references: PMID:1386409
• CR2/CD21 is also the receptor for Epstein-Barr virus, mediating viral entry into host cells.
• The protein is localized at the cell membrane and is expressed on mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes, and follicular dendritic cells of the spleen.
• CR2/CD21 undergoes alternative splicing and contains multiple Sushi (CCP/SCR) domains; it is a glycoprotein with disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 300–590, which includes part of the extracellular domain; consider that the antibody may detect multiple isoforms produced by alternative splicing. Validate in relevant cell lines.
• For Western blot, expect a major band around 113 kDa; however, due to glycosylation and alternative splicing, additional bands may appear. Optimize sample preparation and gel conditions.
• For IHC-P and IF-P, target membrane localization requires optimized fixation and permeabilization. Test with known positive tissues such as spleen, tonsil, or B-cell lymphomas.
• In ELISA, ensure proper coating of recombinant protein or native antigen and include appropriate negative and positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including this CR2/CD21 polyclonal antibody, supporting research on complement pathways, B-cell malignancies, and EBV-related diseases. We offer validated antibody pairs, bulk polyclonal and monoclonal antibodies, ancillary reagents, and custom sourcing for rare targets to streamline your diagnostic development. With end-to-end solutions from concept to clinic, CamelBio is your trusted partner for reliable, high-quality immunoreagents.