Anti-CR2/CD21 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P20023

Core Product Specifications and Parameters

Biological Background: CR2/CD21 Function and Localization

• Receptor for complement component C3d, HNRNPU, and IFNA1, mediating antigen uptake by B-cells and enhancing B-cell activation. Related references: PMID:1849076, PMID:21527715, PMID:7753047
• Forms the B-cell coreceptor complex with CD19, FCRL5, and CD81, crucial for B-cell activation and signaling. Related references: PMID:1383329, PMID:30107486
• Triggers intracellular signaling via SRC kinase, leading to AKT and GSK3 activation independently of BCR/CD19. Related references: PMID:12938232
• Interacts with CD23 (low-affinity IgE receptor), modulating IgE production. Related references: PMID:1386409
• Acts as a receptor for Epstein-Barr virus, facilitating viral entry.
• Localizes to the cell membrane.
• Expressed in mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes, and follicular dendritic cells of the spleen.
• Contains multiple sushi domains; undergoes alternative splicing, glycosylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 933–1033 (C-terminal cytoplasmic region), suggesting the antibody may recognize intracellular epitopes. For Western blot (WB), consider using reducing conditions and validate with appropriate lysates.
• For IHC-P, antigen retrieval protocols (e.g., citrate buffer pH 6.0) may enhance detection; preliminary testing on positive control tissues (such as lymph node or spleen) is recommended.
• Cross-reactivity with mouse and rat is indicated; verify species-specific binding in your model system.
• For ELISA, the recombinant protein used as immunogen can serve as a positive control to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing validated antibodies, ancillary reagents, and specialized procurement for B-cell and complement research. This anti-CR2/CD21 monoclonal antibody supports studies in adaptive immunity, viral entry, and B-cell signaling. Contact us for bulk orders, custom antibody development, or technical consultation.