Anti-CR2/CD21 Monoclonal Antibody for WB, IHC-P, ELISA - P20023

Core Product Specifications and Parameters

Biological Background: CR2/CD21 Function and Localization

• CR2/CD21, also known as Complement receptor type 2, Complement C3d receptor, and Epstein-Barr virus receptor, is a type I membrane glycoprotein.
• It serves as a high-affinity receptor for complement component C3d, attaching to C3d-coated antigens on B-cell surfaces and facilitating antigen recognition and uptake, thereby enhancing B-cell activation and immune responses. Related references: PMID:1849076, PMID:21527715, PMID:7753047
• CR2 forms a B-cell co-receptor complex with CD19, FCRL5, and CD81, which is critical for lowering the threshold of B-cell receptor (BCR) signaling and promoting B-cell activation. Related references: PMID:1383329, PMID:30107486
• Beyond its co-receptor role, CR2 triggers intracellular signaling independently of BCR and CD19 by activating SRC kinase, which phosphorylates nucleolin/NCL, leading to AKT and GSK3 kinase activities. Related references: PMID:12938232
• As a ligand for CD23 (FcεRII), a low-affinity IgE receptor, CR2 participates in regulating IgE production. Related references: PMID:1386409
• CR2 also acts as the receptor for Epstein-Barr virus (EBV) on human B cells, mediating viral entry into host cells.
• The protein is predominantly expressed on the cell membrane of mature B-lymphocytes, and also found on T-lymphocytes, pharyngeal epithelial cells, astrocytes, and follicular dendritic cells of the spleen.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation within the sushi (CCP) domains, characteristic of the complement control protein family.

Experimental Guidance and Technical Tips

• For western blotting, note that the predicted molecular weight of CR2/CD21 is approximately 113 kDa. Consider using a low-percentage SDS-PAGE gel and optimizing transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (paraffin-embedded tissues), antigen retrieval may be necessary; validate in human lymphoid tissues where CR2 is known to be expressed.
• This recombinant monoclonal antibody offers high specificity and can be employed in ELISA-based assays for detecting human CR2/CD21; consider appropriate blocking and detection protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality reagents for complement system and B-cell biology studies. Our portfolio includes validated monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents, as well as rare target sourcing capabilities to accelerate your assay development. Whatever your CR2/CD21-related research or diagnostic need, CamelBio is positioned to supply the critical raw materials that move your projects from concept to clinic.