Anti-CPXM1 Polyclonal Antibody for WB and ELISA - Q96SM3

Core Product Specifications and Parameters

Biological Background: CPXM1 Function and Localization

• CPXM1, also designated metallocarboxypeptidase CPX-1, belongs to the M14 family of zinc-dependent metallocarboxypeptidases, which typically function as proteolytic enzymes but in this case, no carboxypeptidase activity has been observed.
• The protein harbors characteristic structural elements including a signal peptide, multiple disulfide bonds, and N-linked glycosylation motifs, indicating its processing through the endoplasmic reticulum and Golgi for secretion.
• Subcellular localization data categorizes CPXM1 as a secreted protein, implying its presence in the extracellular milieu where it may participate in modulating cell-cell interactions.
• Although CPXM1 contains a conserved zinc-binding domain typical of metalloproteases, functional studies suggest it lacks catalytic activity, possibly serving a non-enzymatic role in tissue organization.
• The CPXM1 gene encodes a protein with a predicted molecular mass of approximately 81.7 kDa, with the recombinant antibody detecting a band at around 82 kDa, close to this theoretical weight.

Experimental Guidance and Technical Tips

• Given that the immunogen spans a large C-terminal region (amino acids 435-734), the antibody likely recognizes the full-length CPXM1 protein; confirm specificity by using siRNA knockdown or knockout cell lines if available.
• For Western blotting, load 20-30 μg of cell lysate and use a 1:500-1:2000 dilution of the primary antibody, optimizing for your specific samples; note that CPXM1 glycosylation may cause a slightly higher apparent molecular weight.
• For ELISA applications, consider coating plates with recombinant CPXM1 (full-length or fragment) and test serial dilutions of antibody to establish optimal working concentrations.
• Validate cross-reactivity claims in your own model systems by testing lysates from human and mouse tissues known to express CPXM1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD manufacturers and research laboratories, offering validated antibody reagents like this CPXM1 polyclonal antibody alongside bulk quantities, custom formulations, and logistical support. Whether you require optimized antibody pairs, rare target sourcing, or ancillary raw materials for protease biology and cell adhesion research, our platform streamlines your workflow from concept to clinic. Contact us to discuss how our integrated supply chain can accelerate your diagnostic development needs.