Anti-CPVL Rabbit Polyclonal Antibody for WB, ELISA - Q9H3G5

Core Product Specifications and Parameters

Biological Background: CPVL Function and Localization

• CPVL (probable serine carboxypeptidase CPVL), also known as vitellogenic carboxypeptidase-like protein (VCP-like protein/hVLP), is a member of the serine carboxypeptidase family.
• It is predicted to function as a lysosomal enzyme involved in the digestion of phagocytosed particles, potentially contributing to an inflammatory protease cascade.
• CPVL may also participate in the trimming of peptides for presentation by major histocompatibility complex (MHC) molecules, linking it to antigen processing.
• The protein is specifically expressed in macrophages and is absent from other leukocyte populations, with abundant expression also observed in heart and kidney tissues, and lower levels in spleen, leukocytes, and placenta.
• Post-translational modifications include glycosylation (glycoprotein) and likely processing from a zymogen precursor, as indicated by UniProt keywords.
• The protein contains a signal peptide for targeting to the secretory pathway and is classified under the hydrolase and protease families (keywords: Carboxypeptidase, Hydrolase, Protease).

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using the antibody at a dilution range appropriate for polyclonal antibodies, such as 1:500–1:2000, and validate detection in macrophage lysates or tissues with high CPVL expression (heart, kidney).
• The immunogen spans amino acids 127–476, which avoids the N-terminal signal peptide, potentially enhancing specificity for the mature protein.
• For ELISA, the antibody may be used as a capture or detection reagent; pilot experiments are recommended to optimize coating concentrations and blocking conditions.
• Cross-reactivity with mouse and rat orthologs is expected based on supplier data, making it suitable for studies in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with reliable access to validated antibodies like this anti-CPVL polyclonal antibody.
• Our portfolio encompasses optimized monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents for immunodetection applications, and we specialize in sourcing rare target materials such as lysosomal enzymes and inflammation-related markers.
• For researchers focusing on carboxypeptidase biology or macrophage function, CamelBio ensures streamlined procurement and technical support to accelerate your development pipeline.