Anti-CPT2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P23786

Core Product Specifications and Parameters

Biological Background: CPT2 Function and Localization

• CPT2 encodes the mitochondrial enzyme carnitine O-palmitoyltransferase 2 (CPT II), an essential acyltransferase involved in fatty acid β-oxidation.
• Functions in the mitochondrial matrix to convert acylcarnitines back to acyl-CoA esters, thereby enabling the entry of long-chain fatty acids into the β-oxidation pathway. Related references: PMID:20538056, PMID:24780397
• Additionally mediates the synthesis of acylcarnitines from accumulated acyl-CoA metabolites, highlighting its bidirectional role in cellular lipid homeostasis. Related references: PMID:20538056, PMID:24780397
• Exhibits enzymatic activity with medium-chain (C8–C12) and long-chain (C14–C18) acyl-CoA substrates. Related references: PMID:20538056
• Localizes to the mitochondrial inner membrane, consistent with its role in connecting mitochondrial fatty acid import with β-oxidation.
• Classified under multiple UniProt keywords including acetylation, acyltransferase, fatty acid metabolism, lipid metabolism, disease variant, and transit peptide, reflecting its regulatory and clinical relevance.
• Its deficiency is linked to metabolic disorders such as CPT II deficiency, a recognized disease variant.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide spanning amino acids 420–658 of human CPT2 (NP_000089.1). This region may provide target specificity; researchers should verify reactivity in their specific sample types.
• For WB, consider using a typical low-fat milk blocking solution and validate the observed band at approximately 74 kDa corresponding to the predicted molecular weight of CPT2.
• For IHC-P, antigen retrieval and antibody dilution should be optimized; nuclear and mitochondrial staining patterns may be observed, consistent with mitochondrial inner membrane localization.
• Cross-reactivity is reported for human, mouse, and rat; users working with other species should validate reactivity independently.
• For ELISA, the antibody can be employed as a capture or detection reagent; consider pairing with a compatible detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality antibodies like this CPT2 rabbit pAb, supporting mitochondrial fatty acid oxidation studies. Beyond single antibodies, we supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, helping accelerate your assay development from concept to clinic.