Anti-CPT2 Rabbit Monoclonal Antibody for WB and ELISA - P23786

Core Product Specifications and Parameters

Biological Background: CPT2 Function and Localization

• CPT2 (Carnitine O-palmitoyltransferase 2), also known as CPT1, IIAE4, or CPTASE, is a mitochondrial enzyme encoded by the CPT2 gene (GeneID: 1376).
• It plays an essential role in mitochondrial fatty acid oxidation by reconverting acylcarnitines into acyl-CoA esters, which are subsequently used for beta-oxidation. Related references: PMID:20538056 PMID:24780397
• CPT2 is also involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites, likely as a protective mechanism against lipotoxicity. Related references: PMID:20538056 PMID:24780397
• The enzyme exhibits activity with medium-chain (C8-C12) and long-chain (C14-C18) acyl-CoA esters, indicating a broad substrate range. Related references: PMID:20538056
• CPT2 is localized to the mitochondrial inner membrane, consistent with its function in mitochondrial fatty acid import.
• It contains a transit peptide for mitochondrial targeting and is subject to post-translational modifications including acetylation.
• The protein has a molecular weight of approximately 74 kDa (UniProt: 73.8 kDa) and is part of the reference proteome.
• Mutations in the CPT2 gene are linked to metabolic disorders, underscoring its clinical significance as a disease-associated protein.

Experimental Guidance and Technical Tips

• For Western blotting, expect a single band at ~74 kDa in mammalian cell or tissue lysates; validate in your relevant sample system.
• An ELISA setup may benefit from using the immunizing peptide (amino acids 559–658) as a positive control or standard.
• Given the antibody’s cross-reactivity with human, mouse, and rat, it can be considered for rodent model studies; cross-validation is recommended.
• Optimize blocking conditions and antibody dilution empirically for your specific assay format and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined solution for sourcing IVD raw materials like the CPT2 antibody, which targets mitochondrial fatty acid metabolism. Our portfolio includes validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents—covering everything from common biomarkers to rare targets. Partner with us to accelerate your assay development and clinical translation with reliable, scalable raw-material supply.