Anti-CPT1C Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8TCG5

Core Product Specifications and Parameters

Biological Background: CPT1C Function and Localization

• CPT1C (Carnitine palmitoyltransferase 1C) is a brain and testis-specific palmitoyl thioesterase (alternative names: CATL1, CPT1P, CPTIC, CPTI-B) belonging to the carnitine/acylcarnitine transferase family, but lacks canonical carnitine palmitoyltransferase catalytic activity or exhibits very low levels. Related references: PMID:25751282
• It functions primarily as an endoplasmic reticulum-resident depalmitoylase that modulates AMPA receptor (AMPAR) trafficking to the plasma membrane by depalmitoylating GRIA1. Related references: PMID:30135643
• CPT1C also regulates AMPAR trafficking through malonyl-CoA-dependent interaction with SACM1L, influencing phosphatidylinositol-3-phosphatase activity (By similarity).
• By binding malonyl-CoA, CPT1C couples this metabolite to ceramide levels, a process necessary for proper dendritic spine maturation and contributing to systemic energy homeostasis and appetite control. Related references: PMID:16651524
• Subcellularly, CPT1C localizes to the endoplasmic reticulum membrane, and is additionally found in neuronal cell projections including dendrites and axons.
• Tissue expression is predominantly in brain and testis, with specific presence in motor neurons; mutations in CPT1C are associated with hereditary spastic paraplegia (SPG73), linking it to neurodegeneration.
• Keywords include acyltransferase, hydrolase, lipid metabolism, alternative splicing, and transmembrane domains, reflecting its dual enzymatic classification and disease relevance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 270-400 of human CPT1C, a region expected to be accessible in the native protein; consider this when designing denaturing vs. native applications.
• For Western blotting, the predicted molecular weight is approximately 91 kDa; run appropriate molecular weight markers and validate band specificity using knockout or knockdown controls.
• In immunofluorescence/ICC, the reported subcellular localization to the endoplasmic reticulum and neuronal projections requires optimized permeabilization and fixation; co-staining with organelle markers is recommended.
• ELISA may be used for quantitative detection; however, optimal coating conditions and detection antibodies should be empirically determined for the specific sample matrix.
• The antibody cross-reacts with human, mouse, and rat; for other species, verify sequence homology in the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated B2B partner for IVD raw-material sourcing, connecting diagnostic manufacturers and research institutes with a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like CPT1C—central to neuronal lipid metabolism and neurodegenerative disease research—we facilitate access to rare and specialized raw materials, streamlining your path from concept to clinical application. Whether you need off-the-shelf antibodies or custom production support, our supply chain expertise ensures that your procurement needs are met with reliability and scientific precision.