Anti-CPT1B Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q92523

Core Product Specifications and Parameters

Biological Background: CPT1B Function and Localization

• CPT1B (Carnitine O-palmitoyltransferase 1, muscle isoform) is a mitochondrial outer membrane enzyme that catalyzes the transfer of long-chain acyl groups from CoA to carnitine, a critical step for fatty acid import into mitochondria for β-oxidation.
• It belongs to the carnitine/choline acyltransferase family and is specifically expressed in heart and skeletal muscle, playing a key role in muscle energy metabolism.
• The protein is highly regulated and is a crucial component in lipid metabolism and energy homeostasis.
• Alternative splicing produces isoforms; the canonical form has a molecular weight of ~88 kDa, though a lower band (e.g., 66 kDa) may represent isoforms or processing events.
• Subcellular localization is the mitochondrion outer membrane.
• UniProt keywords: Acyltransferase, Alternative splicing, Fatty acid metabolism, Lipid metabolism, Membrane, Mitochondrion, Mitochondrion outer membrane, Transferase, Transmembrane, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 313–500 of human CPT1B; this region may confer isoform specificity for the muscle isoform.
• Validated applications include WB, IF-P, IHC-P, and ELISA. In WB, the predicted band is ~88 kDa; a 66 kDa band may also be observed, potentially due to isoform variation or post-translational modification.
• For immunofluorescence and immunohistochemistry, consider using positive control tissues such as heart or skeletal muscle, and optimize fixation and permeabilization conditions.
• Cross-reactivity with mouse and rat is reported; verify performance in the relevant sample systems.
• Titrate antibody concentration individually for each application to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material partner offering diagnostic manufacturers, labs, and research institutes validated monoclonal antibodies like this anti-CPT1B rabbit mAb for metabolic and mitochondrial studies. Our supply capabilities include optimized antibody pairs, bulk ancillary reagents, and tailored sourcing of rare targets. Let CamelBio streamline your raw-material procurement so you can focus on accelerating your research and diagnostics development.