Anti-CPT1B Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q92523

Core Product Specifications and Parameters

Biological Background: CPT1B Function and Localization

• Carnitine O-palmitoyltransferase 1, muscle isoform (CPT1B), also known as CPTI-M, is a mitochondrial outer membrane protein that catalyzes the rate-limiting step in long-chain fatty acid oxidation.
• The enzyme transfers the acyl group from long-chain fatty acyl-CoA to carnitine, forming acylcarnitine, which is then transported across the mitochondrial inner membrane for beta-oxidation.
• Alternative splicing of the CPT1B gene yields multiple transcript variants, potentially contributing to tissue-specific regulation of fatty acid metabolism.
• CPT1B is highly expressed in heart and skeletal muscle, but absent in liver and kidney, underscoring its specialized role in muscle energy homeostasis.
• Its subcellular localization to the mitochondrial outer membrane ensures direct access to cytosolic fatty acyl-CoA pools and efficient channeling of substrates into the mitochondria.
• The protein contains transmembrane helices and belongs to the carnitine/choline acyltransferase family, with key roles in lipid metabolism and transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 533-772) of human CPT1B, which may detect full-length and certain alternatively spliced isoforms.
• For Western blotting, a band around 88 kDa is expected; always include appropriate positive and negative controls from relevant tissues.
• Consider using heart or skeletal muscle lysates as positive controls for CPT1B expression, and liver or kidney lysates as negative controls.
• In immunofluorescence and immunohistochemistry, mitochondrial staining patterns should be observed; co-localization with mitochondrial markers can confirm specificity.
• When using ELISA, pair with a suitable capture antibody and validate in the sample matrix of interest, preferably using recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with high-quality IVD raw materials for every stage of assay development. For CPT1B-related projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, supporting fatty acid metabolism and mitochondrial research. Our global supply network can also source rare-target raw materials—simply reach out with your specific requirements.