Anti-CPT1A Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P50416

Core Product Specifications and Parameters

Biological Background: CPT1A Function and Localization

• CPT1A, the liver isoform of carnitine O-palmitoyltransferase I (CPT I), catalyzes the transfer of long-chain fatty acid-CoA onto carnitine for mitochondrial import and β-oxidation. Related references: PMID:11350182, PMID:14517221, PMID:16651524
• Beyond canonical acyltransferase activity, CPT1A functions as a lysine succinyltransferase, modifying proteins such as ENO1 to regulate metabolism. Related references: PMID:29425493
• In innate immunity, CPT1A sustains the type I interferon response by recruiting ZDHHC4 to palmitoylate and stabilize the mitochondrial antiviral signaling protein MAVS. Related references: PMID:38016475
• CPT1A plays a key role in hepatic triglyceride metabolism and is involved in inducible regulatory T-cell (iTreg) differentiation when activated by butyryl-CoA.
• The enzyme is localized to the mitochondrial outer membrane, consistent with its function in fatty acid transport and signaling.
• Tissue expression is highest in kidney and heart, with lower levels in liver and skeletal muscle.
• Post-translational modifications include acetylation, nitration, and phosphorylation, and genetic variants are associated with metabolic diseases.

Experimental Guidance and Technical Tips

• For Western blot (WB), use reducing conditions and a protein molecular weight marker to confirm the ~88 kDa band in human, mouse, or rat tissue lysates (e.g., liver, kidney). KO validation confirms specificity; a knockout control cell line is recommended for verification.
• For immunohistochemistry (IHC-P), optimize antigen retrieval (e.g., citrate buffer, pH 6.0) and expect a punctate cytoplasmic/mitochondrial staining pattern. Include isotype and tissue controls.
• For ELISA, the recombinant protein immunogen suggests the antibody may serve as a capture or detection reagent. Validate pairing and sensitivity with a recombinant CPT1A standard curve.
• Consider cross‑adsorbing secondary antibodies to minimize species cross‑reactivity when working with multi‑species samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD raw materials, providing diagnostic developers and researchers with one-stop access to validated antibodies, antigen proteins, and ancillary reagents. For metabolic disease and mitochondrial research, we supply high-quality anti‑CPT1A rabbit monoclonal antibodies in bulk, along with rare target sourcing and custom technical services, helping accelerate assay development from discovery to clinical application.