Anti-CPT1A Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, IP, ELISA - P50416

Core Product Specifications and Parameters

Biological Background: CPT1A Function and Localization

• Gene and protein identity: CPT1A encodes carnitine O-palmitoyltransferase 1, liver isoform (CPT I, CPTI-L), a key enzyme in fatty acid metabolism. Alternative names include CPT1, CPT1-L, and L-CPT1.
• Core catalytic function: Mediates the transfer of long-chain fatty acyl groups from CoA to carnitine, a critical step for mitochondrial import and subsequent β-oxidation. Related references: PMID:11350182 PMID:14517221 PMID:16651524
• Lysine succinyltransferase activity: Beyond its canonical acyltransferase role, CPT1A functions as a lysine succinyltransferase, modifying substrates such as ENO1 and influencing metabolism independently of its carnitine acyltransferase function. Related references: PMID:29425493
• Role in immune cell differentiation: Plays a role in inducible regulatory T-cell (iTreg) differentiation upon activation by butyryl-CoA, which relieves malonyl-CoA-mediated repression.
• Regulation of antiviral immunity: Sustains the type I interferon response by recruiting ZDHHC4 to palmitoylate MAVS at mitochondria, thereby stabilizing MAVS and promoting antiviral signaling. Related references: PMID:38016475
• Involvement in liver pathophysiology: Promotes reactive oxygen species (ROS)-induced oxidative stress in liver injury through modulation of NFE2L2 and NLRP3 signaling pathways.
• Subcellular localization: Predominantly located on the mitochondrial outer membrane, consistent with its role in fatty acid transport.
• Tissue expression: Highly expressed in kidney and heart; lower levels in liver and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 520–720 of human CPT1A (NP_001867.2), covering a C-terminal region. Researchers should consider this epitope location when interpreting results from truncated or mutant constructs.
• For western blot, a band of approximately 88 kDa is expected. Validate the band size using appropriate positive controls (e.g., tissues with high CPT1A expression such as kidney or heart) and molecular weight markers.
• When performing immunoprecipitation or ELISA, include relevant controls (e.g., isotype control, lysate from CPT1A‑knockdown cells) to confirm specificity.
• For immunofluorescence and immunohistochemistry, optimize fixation and permeabilization conditions, as mitochondrial membrane proteins may require specific antigen retrieval protocols.
• Although the antibody is validated in human, mouse, and rat, we recommend verifying reactivity in your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and researchers with a comprehensive portfolio of IVD raw materials. This CPT1A rabbit monoclonal antibody supports studies in fatty acid metabolism and mitochondrial biology. Beyond individual reagents, we offer validated antibody pairs, optimized bulk ancillaries, and custom sourcing for rare targets — helping you streamline development from concept to clinic.