Anti-CPSF73 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9UKF6

Core Product Specifications and Parameters

Biological Background: CPSF73 Function and Localization

• CPSF73 (CPSF3) is a 73 kDa subunit of the cleavage and polyadenylation specificity factor (CPSF) complex, essential for pre-mRNA 3'-end formation via recognition of the AAUAAA signal. Related references: PMID:30507380
• It harbors endonuclease activity, directly cleaving pre-mRNA at the poly(A) site to generate the 3'-hydroxyl group for poly(A) tail addition. Related references: PMID:30507380
• CPSF73 is also critical for histone pre-mRNA 3'-end processing, catalyzing U7 snRNP-dependent endoribonucleolytic cleavage and subsequent 5' to 3' exonuclease degradation of the downstream cleavage product. Related references: PMID:30507380
• Required for progression through the S-phase of the cell cycle; also involved in selective miRNA biogenesis during embryonic stem cell differentiation (By similarity). Related references: PMID:30507380
• Contains zinc-binding motifs essential for its nuclease function and undergoes post-translational modifications including acetylation, ubiquitination, and phosphorylation.
• Localizes predominantly to the nucleus, consistent with its role in RNA processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 585-684 of human CPSF73 (Q9UKF6); epitope conservation among human, mouse, and rat is consistent with the observed cross-reactivity.
• For western blot analysis, expect a band at approximately 77 kDa; optimize loading amounts and lysis conditions for your specific samples.
• This monoclonal antibody is suitable for IP and ELISA; titrate the antibody for each application to achieve optimal signal-to-noise ratio.
• Consider using nuclear extract preparations to enrich target detection, given the nuclear localization of CPSF73.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and technical services to support your diagnostic and research projects from development to commercialization. This anti-CPSF73 monoclonal antibody is part of our growing portfolio of validated antibodies, antigens, and ancillary reagents tailored for mRNA processing and epigenetics research. We specialize in sourcing rare targets and providing ready-to-use reagent sets to streamline your assay workflows.