Anti-CPSF6 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q16630

Core Product Specifications and Parameters

Biological Background: Cleavage and Polyadenylation Specificity Factor Subunit 6 (CPSF6) Function and Localization

• CPSF6, also known as CFIm68, is a core subunit of the cleavage factor Im (CFIm) complex, which plays an essential role in pre-mRNA 3'-end cleavage and polyadenylation, a critical step for mRNA maturation.
  Related references: PMID:8626397, PMID:14690600, PMID:29276085
• The CFIm complex binds specifically to UGUA elements upstream of polyadenylation signals and is a master regulator of alternative cleavage and polyadenylation (APA), controlling 3' UTR length and mRNA isoform diversity.
  Related references: PMID:20695905, PMID:23187700, PMID:29276085
• CPSF6 enhances the RNA-binding affinity of its partner NUDT21/CPSF5 to UGUA motifs and promotes RNA looping, thereby directly stimulating the 3'-processing machinery.
  Related references: PMID:15169763, PMID:21295486, PMID:29276085
• Beyond nuclear RNA processing, CPSF6 has been implicated in mRNA export from the nucleus to the cytoplasm.
  Related references: PMID:19864460
• (Microbial infection) CPSF6 interacts with HIV-1 capsid-nucleocapsid complexes and may facilitate viral integration in dividing cells.
• CPSF6 localizes predominantly in the nucleus, including nucleoplasm and nuclear speckles, but can also shuttle to the cytoplasm under certain conditions.
• The CPSF6 gene undergoes alternative splicing, yielding multiple isoforms, and the protein is subject to post-translational modifications such as phosphorylation and methylation, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–330 of human CPSF6 (NP_008938.2). Given the presence of multiple isoforms, consider verifying the detected band size against the expected molecular weight in your sample type.
• This polyclonal antibody has been reported for use in WB, IHC-P, IF/ICC, IP, and ELISA. For IP, pre-clearing steps may optimize capture; for IF, confirm nuclear staining patterns consistent with known localization.
• Suitable for detecting endogenous CPSF6 in human, mouse, and rat samples. Validate cross-reactivity in your specific experimental context if working with less characterized species or tissues.
• For optimal results, titrate antibody concentration for each application and include appropriate positive controls (e.g., HeLa, HEK293 lysates for WB).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by offering validated polyclonal antibodies, like this CPSF6 antibody, for research into mRNA processing and gene expression regulation. Our integrated supply chain covers antibody pairs, bulk ancillary reagents, and custom rare-target sourcing, supporting diagnostic developers and research laboratories from discovery through assay development. Whether you need single-vial access or large-scale production lots, CamelBio connects you with reliable, traceable materials to accelerate your projects.