Anti-Cpsf4l Polyclonal Antibody for WB, ELISA - A6NMK7

Core Product Specifications and Parameters

Biological Background: Putative cleavage and polyadenylation specificity factor subunit 4-like protein Function and Localization

• Encoded by the CPSF4L gene, the human protein A6NMK7 is a putative cleavage and polyadenylation specificity factor subunit 4-like protein, classified under UniProtKB reviewed (Swiss-Prot).
• Predicted to contain zinc finger domains and RNA-binding motifs, indicating potential roles in metal ion chelation and nucleic acid interaction.
• Belongs to the CPSF4-like family, named for its similarity to cleavage and polyadenylation specificity factor subunit 4, which functions in pre-mRNA 3'-end processing.
• Listed as a reference proteome component and identified by proteomics methods, confirming its expression in human cells.
• The mouse ortholog Cpsf4l (Gene ID 52670) shares high sequence similarity and is the target of this polyclonal antibody.
• Subcellular localization and detailed functional characterization remain to be experimentally determined.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant mouse Cpsf4l protein; thus, the polyclonal antibody is expected to recognize multiple epitopes and can be considered for both denaturing (WB) and native (ELISA) applications.
• In Western blot analysis of mouse tissue or cell lysates, detect a specific band at approximately 21 kDa. Use relevant positive (e.g., transfected cell lysate) and negative (e.g., knockout or siRNA-treated) controls to confirm specificity.
• For ELISA, the recombinant protein used as immunogen may serve as a coating antigen or standard. Optimize coating conditions and antibody dilution empirically.
• Cross-reactivity with species other than mouse has not been evaluated. If testing in other organisms, perform a pilot experiment to verify recognition.
• Shipping is on ice bags; upon receipt, store at recommended conditions (typically -20°C or -80°C) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-Cpsf4l polyclonal antibody as a key resource for mRNA processing and gene regulation studies, seamlessly integrated into our comprehensive IVD raw-material portfolio. Our platform delivers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target raw-material procurement, ensuring diagnostic manufacturers and researchers have reliable access to critical tools. From concept to clinic, we streamline your development with quality-assured products and dedicated technical expertise.