Anti-CPSF3L Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q5TA45

Core Product Specifications and Parameters

Biological Background: CPSF3L (INTS11) Function and Localization

• CPSF3L, also known as INTS11, is the catalytic RNA endonuclease subunit of the integrator complex, a multiprotein assembly that mediates promoter-proximal transcription termination of RNA polymerase II (PubMed:16239144, PubMed:25201415, PubMed:28396433). Related references: PMID:16239144 PMID:25201415 PMID:28396433
• The integrator complex enforces a quality checkpoint during early elongation: INTS11 degrades nascent RNA, while associated phosphatases dephosphorylate the Pol II CTD and SPT5, triggering polymerase release (PubMed:32697989, PubMed:33243860, PubMed:33548203). Related references: PMID:32697989 PMID:33243860 PMID:33548203
• The complex also terminates non-coding transcripts, including enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs, and long non-coding RNAs (lncRNAs), thereby regulating genome-wide expression (PubMed:16239144, PubMed:22252320, PubMed:26308897). Related references: PMID:16239144 PMID:22252320 PMID:26308897
• CPSF3L/INTS11 localizes predominantly to the nucleus but is also found in the cytoplasm.
• It harbors a metallonuclease domain that requires zinc and metal ion coordination for its hydrolytic activity, classifying it as a metal-dependent hydrolase.
• Alternative splicing generates multiple protein isoforms, and the protein undergoes post-translational modifications including ubiquitin-like (Ubl) conjugation and isopeptide bond formation.
• Mutations in INTS11 are linked to intellectual disability, underscoring its importance in neurological development.
• Additionally, as part of the integrator complex, INTS11 mediates recruitment of cytoplasmic dynein to the nuclear envelope, a function critical for nuclear dynamics (PubMed:23904267). Related references: PMID:23904267

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 331–600 of human CPSF3L; this region may be conserved across reported isoforms. Consider verifying reactivity in your specific sample system.
• For Western blotting, the predicted molecular weight is approximately 68 kDa (67.6 kDa according to UniProt). Use appropriate loading controls and validate detection in lysates from human, mouse, or rat cells.
• This polyclonal antibody may be suitable for immunoprecipitation (IP) and ELISA; if using for immunofluorescence (IF/ICC), note that CPSF3L/INTS11 exhibits both nuclear and cytoplasmic localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, ancillary reagents, and rare-target sourcing to support your assay development. For researchers and manufacturers working on transcriptional regulation and integrator complex biology, this anti-CPSF3L antibody serves as a reliable tool, and CamelBio can further supply bulk quantities and complementary raw materials to accelerate your project from concept to clinic.