Anti-CPSF2 Polyclonal Antibody for WB, ELISA - Q9P2I0

Core Product Specifications and Parameters

Biological Background: CPSF2 Function and Localization

• CPSF2, also known as CPSF100, is the 100 kDa subunit of the cleavage and polyadenylation specificity factor (CPSF) complex.
• It plays a critical role in pre-mRNA 3'-end processing by recognizing the AAUAAA polyadenylation signal and facilitating cleavage and poly(A) tail addition.
• CPSF2 interacts with poly(A) polymerase and other processing factors to execute efficient mRNA maturation.
• It is also involved in the specialized processing of histone pre-mRNA 3' ends.
• The protein is localized to the nucleus, consistent with its function in mRNA processing.
• CPSF2 is a phosphoprotein with RNA-binding capacity, as indicated by structural and proteomic analyses.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human, mouse, or rat cell lines, with the expected band around 88 kDa. Depending on the sample and gel conditions, you may observe additional bands due to post-translational modifications or isoforms.
• When performing ELISA, using the recombinant immunogen (aa 450-700) as a coating antigen or capture standard may help optimize assay conditions.
• Given the nuclear localization of CPSF2, enriching nuclear extracts could improve detection sensitivity in WB.
• As a polyclonal antibody, lot-to-lot consistency should be verified for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your diagnostic and research workflows with a comprehensive portfolio of IVD raw materials, including polyclonal antibodies like this anti-CPSF2 reagent. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets for mRNA processing studies, our one-stop platform is designed to accelerate your development from concept to clinic.