Anti-CPSF1 Rabbit Polyclonal Antibody for WB, ELISA - Q10570

Core Product Specifications and Parameters

Biological Background: CPSF1 Function and Localization

• CPSF1 encodes the 160 kDa subunit of the cleavage and polyadenylation specificity factor (CPSF) complex, a critical component of the pre-mRNA 3'-end processing machinery. Related references: PMID:14749727
• The CPSF complex recognizes the AAUAAA polyadenylation signal and coordinates cleavage and poly(A) tail addition by interacting with poly(A) polymerase and other factors. Related references: PMID:14749727
• Beyond its role in mRNA processing, CPSF1 may contribute to eye morphogenesis and the development of retinal ganglion cell projections to the midbrain (By similarity).
• Subcellular localization is predominantly nuclear, with enrichment in the nucleoplasm, aligning with its function in pre-mRNA processing.
• Expression is widespread but particularly high in the retina, supporting both its general and tissue-specific roles.
• CPSF1 is classified as a phosphoprotein and contains RNA-binding domains, indicating regulation by phosphorylation and direct RNA interaction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (residues 700–950) of human CPSF1; consider potential epitope accessibility and domain functions when designing experiments.
• For Western blotting, the predicted molecular weight is ~161 kDa; use adequate SDS-PAGE separation and include relevant positive and negative controls to confirm specificity.
• In ELISA applications, validate the antibody with the recombinant immunogen or native protein, and optimize coating and detection conditions for your assay format.
• Cross-reactivity with mouse CPSF1 is reported; verify performance in your specific mouse strain or model before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement with a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This anti-CPSF1 polyclonal antibody supports research into mRNA processing and gene expression regulation, critical areas in diagnostic development. For bulk orders, customized specifications, or end-to-end technical consultation, our team provides reliable sourcing solutions from concept to clinic.