Anti-CPS1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P31327

Core Product Specifications and Parameters

Biological Background: CPS1 Function and Localization

• CPS1 (Carbamoyl-phosphate synthase [ammonia], mitochondrial) is a key urea cycle enzyme that catalyzes the ATP-dependent synthesis of carbamoyl phosphate from ammonia and bicarbonate, essential for ammonia detoxification in ureotelic animals.
• The protein is primarily expressed in the liver and small intestine, consistent with its central role in nitrogen metabolism.
• CPS1 localizes predominantly to the mitochondrial matrix, but has also been detected in the nucleus, nucleolus, and cell membrane, suggesting additional non-canonical functions.
• Post-translational modifications including acetylation, phosphorylation, and glycosylation are reported, which may regulate enzyme activity or localization.
• CPS1 is an allosteric enzyme and a member of the ligase family, containing ATP-binding sites critical for its catalytic function.
• Alternative splicing yields multiple isoforms, and mutations in CPS1 cause carbamoyl phosphate synthetase I deficiency, a severe urea cycle disorder.
• A resolved 3D structure provides insights into the enzyme's domain architecture and regulatory mechanisms.
• The mature protein has a molecular weight of approximately 165 kDa (UniProt: 164,939 Da).

Experimental Guidance and Technical Tips

• The immunogen covers the central region (aa 750-970) of human CPS1, targeting an area potentially conserved across species; verify cross-reactivity in your specific sample system.
• For Western blot (WB), anticipate a band around 165 kDa; use appropriate molecular weight markers and consider loading controls such as GAPDH or beta-actin for normalization.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to detect mitochondrial localization; co-stain with mitochondrial markers if needed.
• In ELISA applications, titrate the antibody and use a suitable coating antigen; the recombinant immunogen may serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with one-stop access to validated IVD raw materials, including this anti-CPS1 antibody that supports urea cycle and metabolic disease research. We offer optimized monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents to streamline your assay development. For rare or difficult targets, our dedicated sourcing team can help secure high-quality raw materials, accelerating your path from concept to clinic.