Anti-CPS1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P31327

Core Product Specifications and Parameters

Biological Background: CPS1 Function and Localization

• CPS1 (Carbamoyl-phosphate synthase [ammonia], mitochondrial), also known as CPSase I, is encoded by the CPS1 gene with aliases PHN, GATD6, CPSASE1.
• It is a key mitochondrial enzyme in the urea cycle, catalyzing the ATP-dependent synthesis of carbamoyl phosphate from ammonia and bicarbonate, thus essential for detoxifying ammonia in ureotelic animals.
• Subcellular localization extends beyond mitochondria; CPS1 is also detected in the nucleus, nucleolus, and cell membrane, suggesting additional non-canonical roles.
• Expression is primarily tissue-specific, with highest levels in the liver and small intestine, aligning with its urea cycle function.
• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, and glycosylation, which may regulate its activity and stability.
• Structural features include ATP-binding domains and allosteric regulation; it contains repeat regions and exhibits alternative splicing.
• Clinically, mutations in CPS1 cause carbamoyl phosphate synthetase I deficiency, an inborn error of the urea cycle leading to hyperammonemia.
• Keywords from UniProt highlight its roles as a ligase, phosphoprotein, and glycoprotein, with proteomics identification confirming its expression.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 820-930 of human CPS1) lies within a region that may be conserved; verify sequence homology with your target species before use.
• For Western blotting, the predicted molecular weight is ~165 kDa; consider using a low-percentage gel (e.g., 8%) and optimized transfer conditions for high-molecular-weight proteins.
• In immunocytochemistry/immunofluorescence, expect mitochondrial staining; include organelle markers to confirm localization, and note that nuclear or membrane signals may also occur.
• For ELISA, this antibody can serve as a capture or detection reagent; pairing with a matched antibody pair may improve sensitivity.
• Validate the antibody in your specific sample system (e.g., cell lines expressing CPS1) with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized IVD raw-material supplier, providing diagnostic manufacturers and researchers with antibodies like this anti-CPS1 polyclonal for urea cycle research. Beyond individual immunoreagents, we offer validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development. Partner with CamelBio to bridge the gap from discovery to clinical application with reliable, customizable supply solutions.