Anti-CPS1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P31327

Core Product Specifications and Parameters

Biological Background: CPS1 Function and Localization

• CPS1, also known as Carbamoyl-phosphate synthetase I, is a mitochondrial enzyme that catalyzes the first committed step of the urea cycle, converting ammonia to carbamoyl phosphate, which is essential for ammonia detoxification in ureotelic animals.
• It plays a critical role in removing excess ammonia from cells, primarily in the liver and small intestine, where it is highly expressed.
• The protein localizes to the mitochondrial matrix but has also been detected in the nucleus, nucleolus, and cell membrane, suggesting additional non-canonical functions.
• CPS1 undergoes various post-translational modifications, including acetylation and phosphorylation, and exhibits alternative splicing, which may regulate its activity or localization.
• As an ATP-binding, allosteric enzyme, it belongs to the ligase family and is a key component of the urea cycle, with mutations linked to disease variants.
• The mature protein has a molecular weight of approximately 165 kDa, consistent with its 164939 Da precursor after cleavage of the mitochondrial transit peptide.

Experimental Guidance and Technical Tips

• For Western blotting, target detection at ~165 kDa requires appropriate gel concentration (e.g., 6-8% SDS-PAGE) and extended transfer times due to high molecular weight.
• In immunofluorescence (IF-P) protocols, optimal fixation and permeabilization conditions should be determined to preserve the mitochondrial structure while allowing antibody access.
• For immunohistochemistry (IHC-P), antigen retrieval pH and method should be validated; liver and small intestine tissue sections serve as suitable positive controls based on tissue specificity.
• As the immunogen is a synthetic peptide from residues 100-200, specificity may be confirmed by peptide competition assays or Western blot analysis using CPS1 knockout cell lysates, if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to a comprehensive portfolio of IVD raw materials, including this CPS1 monoclonal antibody for urea cycle and metabolic research. Our sourcing network offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target procurement, enabling seamless transition from concept to clinical application. Partner with us to accelerate your diagnostic development with reliable, one-stop supply solutions.