Anti-CPOX Rabbit Polyclonal Antibody for WB and ELISA - P36551

Core Product Specifications and Parameters

Biological Background: CPOX Function and Localization

• The CPOX gene encodes oxygen-dependent coproporphyrinogen-III oxidase, a mitochondrial enzyme that catalyzes the sixth step in the heme biosynthetic pathway, converting coproporphyrinogen-III to protoporphyrinogen-IX.
• CPOX is expressed as multiple isoforms through alternative splicing, and the protein localizes to the mitochondrial intermembrane space.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity or stability.
• Mutations in CPOX are associated with hereditary coproporphyria, as indicated by the disease variant keyword in UniProt.
• The protein serves as an oxidoreductase and is involved in porphyrin biosynthesis, essential for heme production.
• CPOX has a calculated molecular weight of approximately 50 kDa, consistent with the observed 50 kDa band in Western blotting.

Experimental Guidance and Technical Tips

• Given the immunogen spanning a large portion of CPOX, the polyclonal antibody may recognize multiple epitopes, ensuring sensitivity in both denaturing (WB) and native (ELISA) assays.
• For Western blotting, consider using a loading control near 50 kDa and validate detection in your specific sample type, as cross-reactivity is confirmed for human, mouse, and rat.
• When developing ELISA protocols, perform cross-titration to optimize antibody and antigen concentrations, and include specificity controls to confirm signal.
• Since CPOX is a mitochondrial enzyme, enrichment of mitochondrial fractions during lysate preparation may enhance signal intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, connecting diagnostic manufacturers and research laboratories with validated antibody pairs, bulk reagents, and rare target antibodies. This anti-CPOX polyclonal antibody supports heme biosynthesis and mitochondrial research applications. Our platform simplifies procurement from discovery to clinical translation, ensuring reliable supply and technical consultation.