Anti-CPLX2 Polyclonal Antibody for WB, ELISA - Q6PUV4

Core Product Specifications and Parameters

Biological Background: CPLX2 Function and Localization

• Complexin-2 (CPLX2), also known as Complexin II and Synaphin-1, is a small 15 kDa coiled-coil protein belonging to the complexin/Synaphin family of SNARE regulators.
• It plays a dual role in exocytosis: it negatively regulates spontaneous synaptic vesicle clustering at the active zone, while positively regulating a late step in evoked neurotransmitter release.
• Beyond synaptic vesicles, CPLX2 is involved in the exocytosis of other cytoplasmic vesicles and mast cell degranulation, indicating broader secretory functions.
• CPLX2 localizes to the cytosol, presynapse, nucleus, and perikaryon of postmitotic neurons, reflecting its dynamic roles in different cellular compartments.
• In the nervous system, it is predominantly expressed in excitatory neurons of the hippocampus and cerebellum, highlighting its importance in synaptic transmission.
• Expression of CPLX2 is down-regulated in brain cortex of patients with Huntington disease, bipolar disorder, or major depression, and in cerebellum of schizophrenia patients, linking it to the pathophysiology of neuropsychiatric disorders.
• The protein features a coiled-coil domain and phosphorylation sites that likely regulate its interaction with the SNARE complex and synaptic function.

Experimental Guidance and Technical Tips

• This antibody is raised against full-length human CPLX2, so it may detect the target across multiple applications; consider titrating for optimal signal in your specific assay.
• For Western blot, expect a band near 15 kDa; use brain tissue lysates (human, mouse, or rat) as positive controls given the nervous system-specific expression.
• In ELISA, consider using the antibody as a capture or detection reagent with appropriate recombinant CPLX2 protein standards to establish assay sensitivity.
• Cross-reactivity with mouse and rat suggests sequence conservation; validate in the relevant species and tissue context before large-scale studies.
• Since CPLX2 is down-regulated in certain neurological disorders, include appropriate disease-state samples when designing translational experiments.

CamelBio: Your One-Stop Sourcing Bridge

This anti-CPLX2 polyclonal antibody is offered by CamelBio as part of a comprehensive portfolio of IVD raw materials for neurological disease and synaptic function research. We supply validated antibody pairs, optimized monoclonals, and bulk ancillary reagents, and can assist with sourcing rare targets like synaptic proteins to accelerate your diagnostic development. From concept to clinic, CamelBio provides one-stop access to raw materials, technical services, and consulting to support your assay development needs.