Anti-CPB1 Rabbit Polyclonal Antibody for WB, ELISA - P15086

Core Product Specifications and Parameters

Biological Background: CPB1 Function and Localization

• CPB1 encodes carboxypeptidase B, a zinc-dependent metalloprotease that cleaves C-terminal basic amino acids (arginine, lysine) from peptides and proteins.
• The protein is synthesized as a zymogen and activated by trypsin; it is secreted from pancreatic acinar cells and stored in zymogen granules.
• Also known as pancreas-specific protein (PASP), reflecting its predominant tissue expression in the pancreas.
• Subcellular localization: secreted into the pancreatic duct and eventually the intestine; within cells, it resides in the lumen of cytoplasmic vesicles (zymogen granules).
• Molecular weight: approximately 47 kDa (predicted 47.4 kDa), consistent with the mature enzyme.
• Keywords highlight its enzymatic function (carboxypeptidase, hydrolase), metal binding (zinc), and presence in cytoplasmic vesicles and secretion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 111-417 of human CPB1 (NP_001862.2), covering a large internal region; consider that it may recognize both pro- and active forms.
• For Western blot (WB), test a range of antibody dilutions using pancreatic tissue or cell line lysates (human, mouse, rat) expressing CPB1; a band near 47 kDa is expected.
• For ELISA, validate using recombinant CPB1 protein or natural samples; ensure appropriate coating conditions and detection systems.
• Cross-reactivity with mouse and rat CPB1 is indicated; however, the degree of reactivity may vary; validate in each species' relevant sample system.
• As a polyclonal antibody, lot-to-lot consistency should be verified with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For pancreatic disease or digestive enzyme-related IVD kit development, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies like this CPB1 pAb, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us to streamline your assay development pipeline with reliable, high-quality raw materials.