Anti-CPA4 (Carboxypeptidase A4) Polyclonal Antibody for WB, ELISA - Q9UI42

Core Product Specifications and Parameters

Biological Background: CPA4 Function and Localization

• CPA4, also known as carboxypeptidase A3, is a secreted zinc-dependent metalloprotease that preferentially cleaves hydrophobic C-terminal residues (Phe, Leu, Ile, Met, Tyr, Val) from peptide substrates. Related references: PMID:20385563
• It is implicated in peptide hormone and neuropeptide catabolism, potentially modulating signaling pathways. Related references: PMID:20385563
• Expression occurs during fetal development in adrenal gland, brain, heart, intestine, kidney, liver, and lung; imprinting leads to preferential maternal allele expression except in the brain.
• The protein has a molecular weight of approximately 47 kDa and contains a signal peptide, zinc-binding catalytic domain, and disulfide bonds crucial for its structure and activity.
• CPA4 is synthesized as an inactive zymogen that requires proteolytic processing, and it undergoes glycosylation.
• Alternative splicing generates multiple isoforms, as indicated by UniProt annotations.

Experimental Guidance and Technical Tips

• The immunogen (residues 114–280) covers a conserved region of the catalytic domain, supporting cross-reactivity with mouse and rat CPA4.
• For Western blot, expect a band near 47 kDa under reducing conditions; glycosylation or incomplete processing may cause slight shifts.
• Use fetal tissue lysates or cell culture supernatants from CPA4-expressing cells as positive controls.
• In ELISA applications, pairing with a capture antibody against a different epitope may improve sensitivity; validate with recombinant protein standards.
• As a secreted protease, CPA4 may require concentration steps for detection in biological fluids.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive selection of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. With expertise in proteases like carboxypeptidase A4, CamelBio facilitates procurement of rare-target antibodies and customized solutions, supporting projects from early research to clinical application.