Anti-CPA3 Polyclonal Antibody for WB, ELISA - P15088

Core Product Specifications and Parameters

Biological Background: CPA3 Function and Localization

• CPA3 encodes mast cell carboxypeptidase A, a zinc-dependent metalloprotease that catalyzes the cleavage of C-terminal amino acids from peptides and proteins.
• It belongs to the carboxypeptidase family and contains key structural features such as disulfide bonds that stabilize its active conformation.
• The protein is synthesized as an inactive zymogen precursor with a signal peptide, requiring proteolytic processing to become fully active.
• Subcellular localization studies place CPA3 within cytoplasmic secretory vesicles of mast cells, aligning with its role in regulated exocytosis during degranulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 110-417, covering the mature catalytic domain; this design may allow detection of both the zymogen and activated forms.
• For Western blot analysis under reducing conditions, consider a typical band around 49kDa; pre-validate with positive controls such as mast cell lines or relevant tissues.
• In ELISA applications, the antibody can be used for antigen capture or detection; optimize coating and detection conditions using recombinant CPA3 or natural sources.
• Given predicted cross-reactivity with mouse and rat, pilot experiments in these species are recommended to confirm specificity and sensitivity in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and life-science manufacturers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. For targets like CPA3 involved in mast cell biology and inflammatory research, we offer reliable sourcing of high-quality raw materials and can assist with custom procurement of rare or specialized reagents. From discovery to production, we provide the integrated supply solutions needed to accelerate your assay development.