Anti-CPA1 Rabbit Polyclonal Antibody for WB, IF, ELISA - P15085

Core Product Specifications and Parameters

Biological Background: Carboxypeptidase A1 Function and Localization

• Carboxypeptidase A1 (CPA1) is a pancreatic metalloprotease that catalyzes the release of C-terminal amino acids, with preference for aromatic or aliphatic residues and little activity towards Asp, Glu, Arg, Lys, or Pro (PubMed:20385563, PubMed:8806703). Related references: PMID:20385563 PMID:8806703
• Converts leukotriene C4 to leukotriene F4 via amide bond hydrolysis (By similarity).
• Contains a zinc ion in its active site and belongs to the M14 metallocarboxypeptidase family.
• Synthesized as an inactive zymogen (pro‑CPA1) requiring proteolytic cleavage for activation.
• Secreted into the extracellular space and found in pancreatic juice.
• Implicated in digestive protein processing and leukotriene metabolism, with potential roles in inflammation.
• Human CPA1 gene (GeneID: 1357) encodes a 419‑amino acid preproprotein; mature active enzyme has a molecular mass of ~47 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1‑100 of human CPA1. This N‑terminal region is predicted to be accessible and should detect both pro‑ and mature forms.
• The antibody is validated for Western blot (WB), immunofluorescence on paraffin sections (IF‑P), and ELISA. For WB, consider a loading control around 47 kDa and validate in pancreas or relevant cancer cell lines.
• Cross‑reactivity with mouse and rat CPA1 is indicated; however, sequence alignment of the immunogen region is recommended to confirm epitope conservation.
• For ELISA applications, pairing with a detection antibody against a C‑terminal epitope may improve sensitivity for mature CPA1.

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