Anti-COX8A Rabbit Polyclonal Antibody for WB and ELISA - P10176

Core Product Specifications and Parameters

Biological Background: Cytochrome c oxidase subunit 8A Function and Localization

• COX8A encodes cytochrome c oxidase subunit 8A, a nuclear-encoded polypeptide of the mitochondrial respiratory chain complex IV (also known as Cytochrome c oxidase polypeptide VIII-liver/heart and COX8-2).
• As a component of cytochrome c oxidase (complex IV), it catalyzes the terminal reduction of oxygen to water in the electron transport chain, coupling electron transfer to proton translocation across the inner mitochondrial membrane to drive ATP synthesis.
• The COX8A gene belongs to a family of nuclear-encoded subunits that structurally modulate the catalytic core of complex IV; alternative isoforms may arise from distinct genes such as COX8C.
• The protein localizes to the mitochondrial inner membrane via a transmembrane helix; it is synthesized as a precursor with an N-terminal transit peptide that is cleaved upon mitochondrial import to yield the mature ~8 kDa form.
• Ubiquitination (Ubl conjugation) has been identified as a post-translational modification, potentially regulating COX8A stability or assembly into the complex.
• Mutations in COX8A are linked to primary mitochondrial disease, specifically mitochondrial complex IV deficiency nuclear type 15 (MC4DN15), highlighting its essential role in oxidative phosphorylation.
• Widely expressed across human tissues, COX8A supports the housekeeping function of mitochondrial respiration in diverse cell types.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 69 amino acids of human COX8A; consider using mitochondrial-enriched lysates to improve detection sensitivity for this low-abundance inner membrane protein.
• For Western blotting, use a high-percentage gel (e.g., 15% or gradient) suitable for resolving ~8 kDa proteins, and validate the band against the predicted molecular weight (UniProt: 7579 Da) with mitochondrial markers.
• In ELISA applications, the polyclonal nature allows recognition of multiple epitopes; perform checkerboard titrations to determine optimal antibody and antigen coating concentrations.
• Cross-reactivity with human and mouse is indicated; for other species, align the immunogen sequence with the target protein to predict reactivity before experimental use.
• Given the mitochondrial localization, pre-treating samples with mild detergents may improve epitope accessibility, though empirical testing is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering a comprehensive portfolio from validated antibodies and recombinant antigens to tailored sourcing solutions. This Anti-COX8A rabbit polyclonal antibody supports research into mitochondrial diseases and oxidative phosphorylation, enabling assay development for complex IV deficiency diagnostics. For bulk requirements or custom reagent services, contact our team to streamline your project from concept to clinic.