Anti-COX7A2L Rabbit Polyclonal Antibody (KD Validated) for WB and ELISA - O14548

Core Product Specifications and Parameters

Biological Background: COX7A2L Function and Localization

• COX7A2L, also referred to as SCAF1, COX7AR, or EB1, is a mitochondrial protein that functions as a critical assembly factor for respiratory chain supercomplexes (respirasomes).
• It acts as an assembly factor that mediates the formation of mitochondrial respiratory supercomplexes, thereby promoting oxidative phosphorylation and energy metabolism.
  Related references: PMID:27545886 PMID:30428348 PMID:33727070
• COX7A2L serves as a molecular adapter that associates with both mitochondrial respiratory complex III (CIII) and complex IV (CIV), promoting their interaction to form supercomplexes.
  Related references: PMID:27545886 PMID:36198313
• It mediates the formation of supercomplexes such as MCIII₂IV₂ and the CS-respirasome (MCI₁III₂IV₂), but is not involved in the formation of the canonical respirasome (MCI₁III₂IV₁).
  Related references: PMID:27545886 PMID:30428348
• Supercomplexes mediated by COX7A2L are required to maintain oxidative phosphorylation under low oxygen conditions and to promote metabolic rewiring toward glycolysis, playing a key role in cellular oxygen adaptation.
  Related references: PMID:36198313
• COX7A2L is localized to the mitochondrial inner membrane and contains a transmembrane helix. It undergoes post‑translational modifications such as acetylation, as identified by proteomics.
• The protein has a calculated molecular weight of approximately 12.6 kDa and migrates at around 13 kDa on SDS‑PAGE.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein encompassing the full‑length human COX7A2L (residues 1–114). It is suitable for Western blot (WB) and ELISA applications.
• For WB, consider using mitochondrial‑enriched fractions from human cell lines or tissues. The expected band size is approximately 13 kDa. Due to the polyclonal nature, it may exhibit robust sensitivity, but validation in the specific experimental system is recommended.
• For ELISA, the antibody can be used for antigen detection; optimization of coating antigen concentration and detection conditions may improve performance.
• As the immunogen covers the entire mature sequence, the antibody is expected to recognize both native and denatured forms of COX7A2L.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive one‑stop sourcing partner for IVD raw materials, offering reliable antibodies like this anti‑COX7A2L polyclonal antibody to support research into mitochondrial function and metabolic diseases. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. We are dedicated to bridging the gap from concept to clinic, helping diagnostic manufacturers and laboratories accelerate their development workflows with high‑quality reagents.