Anti-COX7A2L Rabbit Polyclonal Antibody for WB, ELISA - O14548

Core Product Specifications and Parameters

Biological Background: COX7A2L Function and Localization

• COX7A2L (SCAF1) is a mitochondrial inner membrane protein that functions as an assembly factor for respiratory supercomplexes, promoting oxidative phosphorylation and energy metabolism (PubMed:27545886, PubMed:30428348, PubMed:33727070). Related references: PMID:27545886 PMID:30428348 PMID:33727070
• It acts as a molecular adapter linking complex III and complex IV, mediating the formation of supercomplexes like MCIII2IV2 and the CS-respirasome MCI1III2IV2 (PubMed:27545886, PubMed:30428348, PubMed:36198313). Related references: PMID:27545886 PMID:30428348 PMID:36198313
• It is not required for the canonical MCI1III2IV1 respirasome (By similarity).
• COX7A2L-mediated supercomplexes are critical for cellular adaptation to low oxygen, preventing metabolic exhaustion by maintaining oxidative phosphorylation and promoting glycolysis (PubMed:36198313). Related references: PMID:36198313
• The protein contains a predicted transit peptide and a transmembrane helix, consistent with its localization to the mitochondrial inner membrane.
• Also known as EB1, SIG81, COX7AR, COX7RP; belongs to the cytochrome c oxidase subunit 7A family.
• Post-translational modifications include acetylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–114) of human COX7A2L, which includes the mature protein after cleavage of the transit peptide. This design ensures recognition of the functional form.
• For Western blot, the antibody detects a band at approximately 13 kDa (predicted molecular weight of the mature protein is ~12.6 kDa). Validate in mitochondrial-rich samples (e.g., heart, muscle tissues) from human, mouse, or rat.
• For ELISA, the antibody can be used as a capture or detection reagent; optimal dilutions should be determined by the end-user for their specific assay conditions.
• Since the antibody is polyclonal, consider batch-to-batch consistency in long-term studies; validate in your system.
• Cross-reactivity with human, mouse, and rat suggests conserved epitopes in the N-terminal region; potential for use in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials. For mitochondrial research, we offer validated antibodies against respiratory chain components like COX7A2L, along with optimized antibody pairs, recombinant proteins, and ancillary reagents. Our one-stop sourcing streamlines your workflow from discovery to clinical application.