Anti-COX7A2L Monoclonal Antibody for WB, IHC-P, ELISA - O14548

Core Product Specifications and Parameters

Biological Background: COX7A2L Function and Localization

• COX7A2L (Cytochrome c oxidase subunit 7A2-like, mitochondrial) is a mitochondrial inner membrane protein, also known as SCAF1 (Supercomplex Assembly Factor 1), EB1, COX7AR, and COX7RP.
• It functions as an assembly factor that mediates the formation of specific mitochondrial respiratory supercomplexes (respirasomes), promoting oxidative phosphorylation and energy metabolism. Related references: PMID:27545886, PMID:30428348, PMID:36198313
• COX7A2L acts as a molecular adapter that associates with both complex III (CIII) and complex IV (CIV) to facilitate their interaction, leading to the formation of supercomplexes like MCIII₂IV₂ and the CS-respirasome (MCI₁III₂IV₂). Related references: PMID:27545886, PMID:30428348
• It is not involved in the canonical respirasome (MCI₁III₂IV₁); instead, COX7A2L-mediated supercomplexes are crucial for adapting cellular respiration to low oxygen conditions, enabling metabolic rewiring toward glycolysis and preventing metabolic exhaustion. Related references: PMID:36198313
• The protein is subject to acetylation and contains a transmembrane helix for integration into the inner mitochondrial membrane.
• Its calculated molecular weight is approximately 12.6 kDa, consistent with the observed band at ~13 kDa in western blot.

Experimental Guidance and Technical Tips

• The antibody is recombinant and recognizes an immunogen spanning amino acids 1-114 of human COX7A2L, covering the full-length protein. For western blot, expect a band near 13 kDa; use appropriate molecular weight markers and loading controls.
• As a KD-validated antibody, users may consider confirming target specificity via siRNA or CRISPR-mediated knockdown in their cell system.
• In IHC-P, COX7A2L localizes to mitochondria, so expect a punctate cytoplasmic staining pattern; optimization of antigen retrieval may be required.
• For ELISA applications, pair with a suitable detection system and include a recombinant protein standard curve for quantification.

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CamelBio connects diagnostic manufacturers and laboratories with a comprehensive portfolio of IVD raw materials, including high-quality monoclonal antibodies like this anti-COX7A2L rabbit mAb for mitochondrial and bioenergetics research. We support your development pipeline with validated antibody pairs, bulk ancillary reagents, and rare target sourcing—streamlining your path from concept to clinic. Contact us to explore how our one-stop supply capabilities can accelerate your next project.