Anti-COX7A2 Rabbit Polyclonal Antibody for WB and ELISA - P14406

Core Product Specifications and Parameters

Biological Background: COX7A2 Function and Localization

• COX7A2 encodes the mitochondrial cytochrome c oxidase subunit 7A2, also known as COX VIIa-L (liver/heart isoform), a nuclear-encoded component of complex IV.
• As part of cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial electron transport chain, it catalyzes the reduction of molecular oxygen to water, coupling this reaction to proton translocation and ATP synthesis.
• The protein is anchored in the mitochondrial inner membrane and contributes to the structural and functional integrity of the multi-subunit COX complex.
• Post-translational modifications include acetylation, as evidenced by mass spectrometry-based proteomics.
• The crystal structure of COX reveals the organization of its subunits, including COX7A2, highlighting its integration into the respiratory supercomplex.
• Multiple aliases (COX7AL, COXVIIAL, COX7AL1) reflect the gene’s historical nomenclature and the existence of tissue-specific isoforms, with COX7A2 predominantly expressed in liver and heart.

Experimental Guidance and Technical Tips

• For Western blot, due to the small molecular weight (9 kDa), use a high-percentage gel (e.g., 15%) and optimize transfer conditions to prevent protein loss. Validate detection under reducing conditions.
• In ELISA applications, titrate both the coating antigen and the antibody to establish optimal signal-to-noise ratios; include appropriate positive and negative controls.
• The immunogen corresponds to a central region (aa 33-115) of human COX7A2; reactivity is expected against the mature and partially processed forms, but confirm specificity by knockdown or using a blocking peptide if non-specific bands appear.
• Although cross-reactivity is listed for human, sequence homology across mammals suggests potential reactivity with other species — align the target sequence prior to cross-species experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers with bulk quantities of monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents. For mitochondrial and metabolic disease research, we supply critical reagents such as anti-COX7A2 antibodies to accelerate assay development and biomarker validation. From initial concept to clinical application, CamelBio streamlines procurement for reliable, high-performance raw materials.