Anti-COX7A1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P24310

Core Product Specifications and Parameters

Biological Background: COX7A1 Function and Localization

• COX7A1 is a subunit of mitochondrial respiratory complex IV (cytochrome c oxidase), the terminal enzyme of the electron transport chain, which catalyzes the reduction of oxygen to water (By similarity).
• It specifically promotes homodimerization of complex IV, facilitating the formation of mitochondrial respiratory supercomplexes (respirasomes) that enhance enzyme activity (By similarity).
• Highly expressed in brown adipose tissue but is not required for thermogenesis (By similarity).
• Located in the mitochondrial inner membrane.
• Classified as a member of the cytochrome c oxidase subunit VIIa family; alternative names include COX7AH (heart) and COX7AM (muscle) reflecting tissue-specific isoforms.
• Annotated with UniProt keywords including Direct protein sequencing, Membrane, Mitochondrion inner membrane, Oxidoreductase, and Transmembrane helix.
• The protein has a molecular weight of approximately 9 kDa (calculated as 9118 Da from UniProt) and contains a transit peptide for mitochondrial import.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within the N-terminal region (aa 1-79) of human COX7A1; the antibody may recognize both precursor and mature forms.
• For western blot, expect a band around 9 kDa; ensure appropriate gel percentage and transfer conditions for small proteins.
• This antibody is suitable for immunofluorescence on paraffin-embedded sections (IF-P) and immunohistochemistry (IHC-P); consider antigen retrieval optimization for your tissue type.
• Reported cross-reactivity with human, mouse, and rat; validate the antibody in your specific species and sample system.

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CamelBio supports your development of mitochondrial complex IV and oxidative phosphorylation research tools with validated monoclonal antibodies like this anti-COX7A1 clone, along with paired detection reagents, bulk ancillary materials, and rare-target sourcing. Our integrated supply model streamlines procurement from early discovery through assay scale-up. Reach out to explore how we can accelerate your next diagnostic or research project today.