Anti-COX7A1 Polyclonal Antibody for WB, IHC-P, ELISA - P24310

Core Product Specifications and Parameters

Biological Background: COX7A1 Function and Localization

• COX7A1 (Cytochrome c oxidase subunit 7A1, also known as COX7A, COX7AH, COX7AM) is a mitochondrial inner membrane protein and the heart/muscle-specific isoform of subunit VIIa of cytochrome c oxidase.
• It is a component of mitochondrial respiratory complex IV (CIV, cytochrome c oxidase), the terminal enzyme of the electron transport chain that catalyzes the reduction of oxygen to water, driving oxidative phosphorylation.
• COX7A1 acts as an assembly factor that specifically promotes homodimerization of CIV complexes, mediating the formation of mitochondrial respiratory supercomplexes (respirasomes); supercomplexes containing two CIV molecules exhibit improved enzymatic activity.
• The protein is anchored in the mitochondrial inner membrane and contains a transmembrane helix, as indicated by its transit peptide and membrane-associated keywords.
• Annotated keywords include direct protein sequencing, mitochondrion inner membrane, oxidoreductase, proteomics identification, reference proteome, transmembrane, and transit peptide.
• The mature protein has a molecular weight of approximately 9.1 kDa (9 kDa reported), consistent with its small single-pass membrane topology.
• Despite high expression in brown adipose tissue, COX7A1 is not required for thermogenesis, highlighting its primary role in respiratory chain architecture rather than heat production.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length mature protein (amino acids 1–79), so the antibody likely recognizes both the precursor and mature forms; a band near 9 kDa is expected in reducing SDS-PAGE.
• For Western blotting, use high-concentration gels and optimized transfer conditions tailored for low-molecular-weight proteins to avoid loss through membranes.
• In IHC-P, validate the antibody in positive control tissues such as human heart or skeletal muscle, where COX7A1 is most abundant, and consider antigen retrieval methods suitable for mitochondrial inner membrane proteins.
• The antibody’s cross-reactivity with mouse and rat COX7A1 enables translational studies across species; however, confirm performance in your sample matrix and compare with species-specific controls.
• For ELISA, the immunogen is a recombinant fusion protein; ensure the capture/detection setup avoids interference from the fusion tag if the antibody is used in a matched pair.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents. For mitochondrial targets like COX7A1, we can supply optimized polyclonal and monoclonal antibodies, bulk conjugates, and technical consultation to streamline your oxidative phosphorylation or respiratory chain assay development. Let CamelBio be your reliable partner in sourcing rare target reagents and accelerating your diagnostic workflow.