Anti-COX6C Polyclonal Antibody for WB, IF/ICC, ELISA - P09669

Core Product Specifications and Parameters

Biological Background: COX6C Function and Localization

• COX6C encodes subunit 6C of cytochrome c oxidase (complex IV), the terminal enzyme of the mitochondrial respiratory chain.
• Cytochrome c oxidase catalyzes the reduction of molecular oxygen to water, coupling electron transfer to proton pumping across the inner mitochondrial membrane.
• The enzyme contains multiple subunits; COX6C is one of the nuclear-encoded subunits that may modulate catalytic activity and assembly.
• Located in the mitochondrial inner membrane, COX6C contributes to the structural integrity of the supercomplex.
• The protein is small (8.8 kDa) and predicted to contain a transmembrane helix.
• Known structural data (3D-structure, direct protein sequencing) support its integration into the complex IV superassembly.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–75); performance may depend on protein conformation or sample preparation.
• For Western blot, use denaturing conditions to expose the epitope and select lysates with high COX6C expression (e.g., heart, skeletal muscle, liver).
• In IF/ICC, confirm mitochondrial localization by co-staining with a mitochondrial marker.
• Cross-reactivity with mouse has been reported; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined path to IVD raw materials, technical services, and consulting from concept to clinic. For mitochondrial targets like COX6C, we supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and can source rare target raw materials to support your respiratory chain research and metabolic studies.