Anti-COX6B1 Rabbit Polyclonal Antibody for WB, ELISA - P14854

Core Product Specifications and Parameters

Biological Background: COX6B1 Function and Localization

• COX6B1 is a subunit of cytochrome c oxidase (complex IV), the terminal enzyme of the mitochondrial electron transport chain, essential for oxidative phosphorylation.
• Complex IV catalyzes the reduction of molecular oxygen to water, driving proton translocation across the inner mitochondrial membrane and contributing to ATP synthesis.
• The protein is located in the mitochondrial inner membrane, as a multi-pass membrane protein, and is integral to respiratory supercomplex formation.
• Alternative names include COX VIb-1, COXG, COX6B, and MC4DN7; mutations in this gene are associated with mitochondrial complex IV deficiency, a primary mitochondrial disease.
• Post-translational modifications include acetylation and formation of disulfide bonds, which may regulate its activity or stability.
• Experimentally determined molecular weight is approximately 10 kDa, consistent with the predicted mass of 10,192 Da.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant fusion protein covering the N-terminal 1–86 amino acids of human COX6B1; it is expected to recognize both endogenous and recombinant forms in denaturing and native assays.
• For Western blot, we recommend using mitochondrial-enriched lysates from human or mouse cells to enhance detection of the 10 kDa band; initial antibody dilution may need empirical optimization.
• In ELISA applications, this polyclonal antibody can serve as a capture or detection reagent; ensure appropriate blocking and washing conditions to minimize background.
• Given the high conservation of COX6B1 across species, cross-reactivity with human and mouse is supported; validation in the intended sample system is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop partner for IVD raw materials, supporting the entire development pipeline from concept to clinic. This COX6B1 antibody is part of a broad portfolio targeting mitochondrial biology and metabolic disorders. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic assay development.