Anti-COX6B1 Rabbit Polyclonal Antibody for WB, ELISA - P14854

Core Product Specifications and Parameters

Biological Background: COX6B1 Function and Localization

• COX6B1 encodes a subunit of cytochrome c oxidase (complex IV), the terminal enzyme of the mitochondrial electron transport chain, which drives oxidative phosphorylation.
• As a structural component of complex IV, COX6B1 contributes to the reduction of molecular oxygen to water, utilizing electrons from cytochrome c and protons from the mitochondrial matrix.
• The protein is localized to the mitochondrial inner membrane, consistent with its role in the respiratory chain.
• Post-translational modifications include acetylation and the formation of a disulfide bond, which may influence protein stability or function.
• Mutations in COX6B1 are associated with primary mitochondrial disease, highlighting its essential role in cellular energy metabolism.
• COX6B1 is also referred to as COX VIb-1, COXG, COX6B, and MC4DN7, reflecting alternative splicing or nomenclature variations.
• The molecular weight of the mature protein is approximately 10 kDa, consistent with its small subunit size within the oxidase complex.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 10 kDa is expected; consider using high-percentage SDS-PAGE gels for better resolution of small proteins.
• Given the mitochondrial membrane localization, fractionation or mitochondrial enrichment may enhance detection sensitivity in whole cell lysates.
• The antibody cross-reacts with mouse and rat COX6B1, making it suitable for studies in rodent models after appropriate validation.
• For ELISA applications, optimize antibody dilution and pair with a compatible detection system for quantitative analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this COX6B1 rabbit polyclonal antibody for mitochondrial respiratory chain research. Our one-stop sourcing platform provides validated antibodies, bulk ancillary reagents, and customized procurement services to support diagnostic manufacturers from concept to clinic. Partner with CamelBio to secure reliable raw materials for your immunoassay development needs.