Anti-COX6B1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P14854

Core Product Specifications and Parameters

Biological Background: Cytochrome c oxidase subunit 6B1 Function and Localization

• Cytochrome c oxidase subunit 6B1 (COX6B1), also known as COX VIb-1, is a nuclear-encoded accessory subunit of mitochondrial complex IV (cytochrome c oxidase), the terminal enzyme of the electron transport chain. It participates in the final step of oxidative phosphorylation by contributing to the reduction of molecular oxygen to water.
• As a component of the 13-subunit mammalian cytochrome c oxidase, COX6B1 assists in electron transfer from cytochrome c to oxygen, while the complex simultaneously pumps protons from the matrix into the intermembrane space, establishing the proton gradient that drives ATP synthase.
• The catalytic core of complex IV consists of three mitochondrial-encoded subunits (COX1, COX2, COX3), while COX6B1 and other nuclear-encoded subunits are imported from the cytoplasm and are essential for assembly, stability, or regulation of the holoenzyme.
• COX6B1 is an integral membrane protein firmly embedded in the mitochondrial inner membrane, positioning it within the respiratory supercomplex that facilitates efficient electron transfer.
• Post-translational modifications of COX6B1 include acetylation and the presence of a disulfide bond; these may influence its structure and interaction within the complex. Variants in COX6B1 are implicated in primary mitochondrial disease, underscoring its importance in cellular energy metabolism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1–86 of human COX6B1; the antibody is expected to recognize an epitope within this N-terminal region. This design may provide specificity for the COX6B1 isoform.
• For Western blot (WB), the predicted molecular weight of COX6B1 is approximately 10 kDa. Use appropriate gel percentages and transfer conditions for low-molecular-weight proteins, and consider optimizing antibody concentration for your sample type.
• In immunohistochemistry on paraffin sections (IHC-P), antigen retrieval may be necessary. Validate staining patterns in appropriate human tissue controls, as COX6B1 is broadly expressed in mitochondria-rich tissues.
• For ELISA, the antibody can be employed as a detection reagent; however, pairing suitability and optimal dilutions should be empirically determined in your assay system.
• While these suggestions are based on general principles, we recommend thorough validation of the antibody in your specific experimental context.

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