Anti-COX6A1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P12074

Core Product Specifications and Parameters

Biological Background: COX6A1 Function and Localization

• COX6A1, also known as cytochrome c oxidase polypeptide VIa-liver or COX VIa-L, is a subunit of cytochrome c oxidase (complex IV), the terminal enzyme of the mitochondrial electron transport chain.
• Cytochrome c oxidase catalyzes the reduction of molecular oxygen to water, using electrons from reduced cytochrome c. This process couples electron transfer to proton pumping across the inner membrane, contributing to the electrochemical gradient that drives ATP synthesis.
• The COX6A1 gene encodes the liver-specific isoform of subunit VIa, distinguishing it from other tissue-specific isoforms.
• The protein localizes to the mitochondrial inner membrane, where it participates in the formation of the respiratory chain supercomplex.
• A mitochondrial transit peptide directs the precursor protein to the mitochondrion; after import and cleavage, the mature protein adopts a transmembrane helix topology embedded in the inner membrane.
• Keywords associated with COX6A1 include membrane, oxidoreductase, and neurodegeneration, reflecting its role in energy metabolism and its association with disorders like Charcot-Marie-Tooth disease.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 25–109) encompasses a region of the mature COX6A1 protein that is likely highly conserved, supporting cross-reactivity with human, mouse, and rat samples.
• For WB analysis, consider enriching mitochondrial fractions to enhance detection of this low-abundance membrane protein; validate in the appropriate tissue or cell line context.
• IHC-P may benefit from antigen retrieval optimization; include positive control tissues known to express COX6A1 (e.g., liver, heart) and confirm specificity with blocking peptides or knockout controls.
• IF/ICC applications should include co-localization with mitochondrial markers and careful fixation/permeabilization to preserve mitochondrial structure.
• ELISA setups may be used for quantitative detection, but titration of both capture and detection antibodies is recommended to achieve optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For projects targeting COX6A1 and mitochondrial oxidative phosphorylation, we offer a reliable supply of polyclonal antibodies, monoclonal options, and custom sourcing of rare targets—streamlining your path from concept to clinic.